P. zeylanica is perennial herb that distributed extensively in all over Asia and African region in world. Plumbagin is the major constituent derived from the roots of Plumbago zeylanica L. is a naphthoquinone compound. Plumbagin was isolated from P. zeylanica L. root and identified by HPLC, 1H-NMR, 13C-NMR and Mass. The qualitative and quantitative analysis of plumbagin, a major constituent, chemical and biological marker present in P. zeylanica L. done by using HPLC-UV method. The quality of the marketed and self collected samples of Plumbago zeylanica roots were also quantified by HPLC-UV method. The study suggested that there is a lot of difference in the quality of the drug marketed in different parts of country. HPLC-UV analysis showed the higher concentration of plumbagin in the self collected sample of P. zeylanica (0.51 % w/w) than marketed sample (0.20 %). This paper provides anti microbial activity of isolated plumbagin and plumbagin Beta cyclodextrin complex in colonic environmental microbial flora against microorganism strains i.e. E. coli. and Staphylococcus aureus. Antimicrobial activity of plumbagin and its complex was validated using an in-vitro zone of inhibition model. Plumbagin Beta-cyclodextrin complex exhibited more antimicrobial activity than plumbagin.
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